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cwl
Commits
5a226964
Commit
5a226964
authored
Apr 15, 2022
by
Nijsse, Bart
Browse files
change kraken.cwl version and busco fix
parent
b6219b59
Changes
1
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cwl/workflows/workflow_nanopore_assembly.cwl
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5a226964
...
@@ -185,10 +185,10 @@ steps:
...
@@ -185,10 +185,10 @@ steps:
out: [standard_report, sample_report]
out: [standard_report, sample_report]
illumina_kraken2:
illumina_kraken2:
label:
Kraken2 workflow
label:
Taxonomic classification with Kraken2
doc:
Read t
axonomic classification
using the Kraken2
doc:
T
axonomic classification
of FASTQ reads
when: $(inputs.binning)
when: $(inputs.binning)
run: ../
metagenomics/
kraken2/kraken2.cwl
run: ../kraken2/kraken2.cwl
in:
in:
binning: binning
binning: binning
tmp_id: identifier
tmp_id: identifier
...
@@ -198,26 +198,29 @@ steps:
...
@@ -198,26 +198,29 @@ steps:
database: kraken_database
database: kraken_database
forward_reads: workflow_quality_illumina/QC_forward_reads
forward_reads: workflow_quality_illumina/QC_forward_reads
reverse_reads: workflow_quality_illumina/QC_forward_reads
reverse_reads: workflow_quality_illumina/QC_forward_reads
out: [report, kraken]
paired_end:
#############################################
default: true
#### Gzip compression of kraken2 files
out: [standard_report, sample_report]
kraken2_compress:
kraken2_compress:
run: ../bash/pigz.cwl
run: ../bash/pigz.cwl
label: Compress kraken2
doc: Compress large kraken2 report file
scatter: inputfile
scatter: inputfile
in:
in:
inputfile: [nanopore_kraken2/standard_report, illumina_kraken2/
kraken
]
inputfile: [nanopore_kraken2/standard_report, illumina_kraken2/
standard_report
]
threads: threads
threads: threads
out: [outfile]
out: [outfile]
#############################################
#### Visual representation of taxonomic classification
kraken2_krona:
kraken2_krona:
label:
taxonomic classification visualization
label:
Krona Kraken2
doc:
v
isualization of
taxonomic classification
with Krona
doc:
V
isualization of
kraken2
with Krona
run: ../krona/krona.cwl
run: ../krona/krona.cwl
scatter: kraken
scatter: kraken
in:
in:
kraken: [nanopore_kraken2/sample_report, illumina_kraken2/report]
kraken: [nanopore_kraken2/sample_report, illumina_kraken2/
sample_
report]
out: [krona_html]
out: [krona_html]
#############################################
#############################################
#### De novo assembly with Flye
#### De novo assembly with Flye
flye:
flye:
...
@@ -255,7 +258,7 @@ steps:
...
@@ -255,7 +258,7 @@ steps:
#### Workflow Pilon assembly polishing
#### Workflow Pilon assembly polishing
workflow_pilon:
workflow_pilon:
label: Pilon
label: Pilon
doc:
Short
reads assembly polishing with Pilon
doc:
Illumina
reads assembly polishing with Pilon
when: $(inputs.binning)
when: $(inputs.binning)
run: workflow_pilon_mapping.cwl
run: workflow_pilon_mapping.cwl
in:
in:
...
@@ -273,7 +276,7 @@ steps:
...
@@ -273,7 +276,7 @@ steps:
#### Assembly evaluation with QUAST
#### Assembly evaluation with QUAST
metaquast_nanopore_pilon:
metaquast_nanopore_pilon:
label: Illumina assembly evaluation
label: Illumina assembly evaluation
doc: Illumina evaluation of polished assembly with metaQUAST
doc: Illumina evaluation of
pilon
polished assembly with metaQUAST
when: $(inputs.binning)
when: $(inputs.binning)
run: ../metaquast/metaquast.cwl
run: ../metaquast/metaquast.cwl
in:
in:
...
@@ -284,8 +287,8 @@ steps:
...
@@ -284,8 +287,8 @@ steps:
#############################################
#############################################
#### BBmap read mapping (illumina reads) for binning
#### BBmap read mapping (illumina reads) for binning
illumina_pilon_readmapping:
illumina_pilon_readmapping:
label:
BBmap r
ead mapping
label:
R
ead mapping
doc: Illumina read mapping
using BBmap on assembled contigs
doc: Illumina read mapping
on pilon assembly for binning
when: $(inputs.binning)
when: $(inputs.binning)
run: ../bbmap/bbmap.cwl
run: ../bbmap/bbmap.cwl
in:
in:
...
@@ -326,7 +329,7 @@ steps:
...
@@ -326,7 +329,7 @@ steps:
threads: threads
threads: threads
step:
step:
default: 1
default: 1
out: [metabat2_output,checkm_output,gtdbtk_output]
out: [metabat2_output,checkm_output,gtdbtk_output
,busco_output
]
#############################################
#############################################
#### Move to folder if not part of a workflow
#### Move to folder if not part of a workflow
...
@@ -336,7 +339,7 @@ steps:
...
@@ -336,7 +339,7 @@ steps:
run: ../expressions/files_to_folder.cwl
run: ../expressions/files_to_folder.cwl
in:
in:
files:
files:
source: [kraken2_compress/outfile, nanopore_kraken2/sample_report,
kraken2_krona/krona_html,
illumina_kraken2/report]
source: [kraken2_compress/outfile,
kraken2_krona/krona_html,
nanopore_kraken2/sample_report, illumina_kraken2/
sample_
report]
linkMerge: merge_flattened
linkMerge: merge_flattened
destination:
destination:
valueFrom: $("2_Kraken2_classification")
valueFrom: $("2_Kraken2_classification")
...
@@ -470,7 +473,7 @@ steps:
...
@@ -470,7 +473,7 @@ steps:
in:
in:
binning: binning
binning: binning
folders:
folders:
source: [workflow_binning/metabat2_output, workflow_binning/checkm_output, workflow_binning/gtdbtk_output]
source: [workflow_binning/metabat2_output, workflow_binning/checkm_output, workflow_binning/gtdbtk_output
, workflow_binning/busco_output
]
linkMerge: merge_flattened
linkMerge: merge_flattened
pickValue: all_non_null
pickValue: all_non_null
destination:
destination:
...
...
Nijsse, Bart
@nijss011
mentioned in commit
f4ba6c1c
·
Apr 19, 2022
mentioned in commit
f4ba6c1c
mentioned in commit f4ba6c1cb0f5d8a723838fedb5e59b5d6f96b080
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